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e coli strain hst08 takara bio  (TaKaRa)


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    Structured Review

    TaKaRa e coli strain hst08 takara bio
    E Coli Strain Hst08 Takara Bio, supplied by TaKaRa, used in various techniques. Bioz Stars score: 97/100, based on 1482 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/e+coli+hst08+strain/Stellar+Competent+Cells/10__1091_slash_mbc__e24___11___0529-140-0-4
    Average 97 stars, based on 1482 article reviews
    e coli strain hst08 takara bio - by Bioz Stars, 2026-09
    97/100 stars

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    Related Articles

    Plasmid Preparation:

    Article Title: Compositions and methods for making selenocysteine containing polypeptides
    Article Snippet: E. coli HST08 strain (Clontech) was mainly used for plasmid construction. .. E. coli HST08 strain (Clontech) was mainly used for plasmid construction. .. E. coli Stellar strain (Clontech) was mainly used for plasmid construction.

    Article Title: Base sequence for protein expression and method for producing protein using same
    Article Snippet: Next, an E. coli-derived plasmid pMD20 (manufactured by Takara Bio Inc.) was used as a template to obtain a gene fragment derived from the plasmid using primers 13 and 14. .. Next, gene fragment of upstream sequence of ligD gene, gene fragment of its downstream sequence, and gene fragment of plasmid pMD20 were treated with a cloning kit (manufactured by Takara Bio Inc., product name: In-Fusion HD Cloning kit) and used in the transformation of an E. coli HST08 strain (manufactured by Takara Bio Inc.) to construct a plasmid pM-Ao Δ ligD. .. Next, the plasmid pM-Ao Δ ligD was treated with the restriction enzyme SmaI (manufactured by Takara Bio Inc.) at 30° C. and purified using the purification kit to obtain the restriction treatment product of the plasmid pM-Ao Δ ligD (gene fragment).

    Article Title: Base sequence for protein expression and method for producing protein using same
    Article Snippet: .. Next, these 7 gene fragments were sequentially treated with a cloning kit (manufactured by Takara Bio Inc., product name: In-Fusion HD Cloning kit) and used in the transformation of an E. coli HST08 strain (manufactured by Takara Bio Inc.) to construct a plasmid pPT. .. Next, the plasmid pPT was treated with a restriction enzyme SmaI (manufactured by Takara Bio Inc.) at 30° C. and purified using the purification kit to obtain the restriction treatment product of the plasmid (gene fragment).

    Sequencing:

    Article Title: Base sequence for protein expression and method for producing protein using same
    Article Snippet: Next, an E. coli-derived plasmid pMD20 (manufactured by Takara Bio Inc.) was used as a template to obtain a gene fragment derived from the plasmid using primers 13 and 14. .. Next, gene fragment of upstream sequence of ligD gene, gene fragment of its downstream sequence, and gene fragment of plasmid pMD20 were treated with a cloning kit (manufactured by Takara Bio Inc., product name: In-Fusion HD Cloning kit) and used in the transformation of an E. coli HST08 strain (manufactured by Takara Bio Inc.) to construct a plasmid pM-Ao Δ ligD. .. Next, the plasmid pM-Ao Δ ligD was treated with the restriction enzyme SmaI (manufactured by Takara Bio Inc.) at 30° C. and purified using the purification kit to obtain the restriction treatment product of the plasmid pM-Ao Δ ligD (gene fragment).

    Cloning:

    Article Title: Base sequence for protein expression and method for producing protein using same
    Article Snippet: Next, an E. coli-derived plasmid pMD20 (manufactured by Takara Bio Inc.) was used as a template to obtain a gene fragment derived from the plasmid using primers 13 and 14. .. Next, gene fragment of upstream sequence of ligD gene, gene fragment of its downstream sequence, and gene fragment of plasmid pMD20 were treated with a cloning kit (manufactured by Takara Bio Inc., product name: In-Fusion HD Cloning kit) and used in the transformation of an E. coli HST08 strain (manufactured by Takara Bio Inc.) to construct a plasmid pM-Ao Δ ligD. .. Next, the plasmid pM-Ao Δ ligD was treated with the restriction enzyme SmaI (manufactured by Takara Bio Inc.) at 30° C. and purified using the purification kit to obtain the restriction treatment product of the plasmid pM-Ao Δ ligD (gene fragment).

    Article Title: Base sequence for protein expression and method for producing protein using same
    Article Snippet: .. Next, these 7 gene fragments were sequentially treated with a cloning kit (manufactured by Takara Bio Inc., product name: In-Fusion HD Cloning kit) and used in the transformation of an E. coli HST08 strain (manufactured by Takara Bio Inc.) to construct a plasmid pPT. .. Next, the plasmid pPT was treated with a restriction enzyme SmaI (manufactured by Takara Bio Inc.) at 30° C. and purified using the purification kit to obtain the restriction treatment product of the plasmid (gene fragment).

    Transformation Assay:

    Article Title: Base sequence for protein expression and method for producing protein using same
    Article Snippet: Next, an E. coli-derived plasmid pMD20 (manufactured by Takara Bio Inc.) was used as a template to obtain a gene fragment derived from the plasmid using primers 13 and 14. .. Next, gene fragment of upstream sequence of ligD gene, gene fragment of its downstream sequence, and gene fragment of plasmid pMD20 were treated with a cloning kit (manufactured by Takara Bio Inc., product name: In-Fusion HD Cloning kit) and used in the transformation of an E. coli HST08 strain (manufactured by Takara Bio Inc.) to construct a plasmid pM-Ao Δ ligD. .. Next, the plasmid pM-Ao Δ ligD was treated with the restriction enzyme SmaI (manufactured by Takara Bio Inc.) at 30° C. and purified using the purification kit to obtain the restriction treatment product of the plasmid pM-Ao Δ ligD (gene fragment).

    Article Title: Base sequence for protein expression and method for producing protein using same
    Article Snippet: .. Next, these 7 gene fragments were sequentially treated with a cloning kit (manufactured by Takara Bio Inc., product name: In-Fusion HD Cloning kit) and used in the transformation of an E. coli HST08 strain (manufactured by Takara Bio Inc.) to construct a plasmid pPT. .. Next, the plasmid pPT was treated with a restriction enzyme SmaI (manufactured by Takara Bio Inc.) at 30° C. and purified using the purification kit to obtain the restriction treatment product of the plasmid (gene fragment).

    Construct:

    Article Title: Base sequence for protein expression and method for producing protein using same
    Article Snippet: Next, an E. coli-derived plasmid pMD20 (manufactured by Takara Bio Inc.) was used as a template to obtain a gene fragment derived from the plasmid using primers 13 and 14. .. Next, gene fragment of upstream sequence of ligD gene, gene fragment of its downstream sequence, and gene fragment of plasmid pMD20 were treated with a cloning kit (manufactured by Takara Bio Inc., product name: In-Fusion HD Cloning kit) and used in the transformation of an E. coli HST08 strain (manufactured by Takara Bio Inc.) to construct a plasmid pM-Ao Δ ligD. .. Next, the plasmid pM-Ao Δ ligD was treated with the restriction enzyme SmaI (manufactured by Takara Bio Inc.) at 30° C. and purified using the purification kit to obtain the restriction treatment product of the plasmid pM-Ao Δ ligD (gene fragment).

    Article Title: Base sequence for protein expression and method for producing protein using same
    Article Snippet: .. Next, these 7 gene fragments were sequentially treated with a cloning kit (manufactured by Takara Bio Inc., product name: In-Fusion HD Cloning kit) and used in the transformation of an E. coli HST08 strain (manufactured by Takara Bio Inc.) to construct a plasmid pPT. .. Next, the plasmid pPT was treated with a restriction enzyme SmaI (manufactured by Takara Bio Inc.) at 30° C. and purified using the purification kit to obtain the restriction treatment product of the plasmid (gene fragment).

    Cell Culture:

    Article Title: Overexpression of purine nucleoside phosphorylase increases the adenosine content in Ganoderma lucidum
    Article Snippet: .. The E. coli HST08 strain (Takara, Dalian, China) was cultured in Luria-Bertani (LB) medium supplemented with kanamycin (50 μg/mL). ..



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